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Tested Applications
| Positive WB detected in | Insulin treated C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85141-5-RR targets Phospho-NDRG1 (Thr346) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | N-myc downstream regulated 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 46 kDa, 48 kDa |
| GenBank Accession Number | BC006260 |
| Gene Symbol | NDRG1 |
| Gene ID (NCBI) | 10397 |
| RRID | AB_3744048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-NDRG1 (Thr346) antibody 85141-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Phytomedicine FBXO11 enhances colorectal cancer progression through TMBIM6 stabilization: Echinacoside as a natural inhibitor. | ||
Int Immunopharmacol Glucocorticoid signaling mitigates colitis-associated visceral hypersensitivity by suppressing 5-HT release in enterochromaffin cells via a GR-PI3K-SGK1 axis. |
