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Tested Applications
| Positive WB detected in | PDGF treated NIH/3T3 cells, PDGF treated C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81219-2-RR targets Phospho-SHP2 (Tyr580) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein tyrosine phosphatase, non-receptor type 11 |
| Calculated Molecular Weight | 597 aa, 68 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC008692 |
| Gene Symbol | PTPN11 |
| Gene ID (NCBI) | 5781 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q06124 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-SHP2 (Tyr580) antibody 81219-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
