RAB11B-Specific Polyclonal antibody

RAB11B-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19742-1-AP

Synonyms

GTP binding protein YPT3, H YPT3, RAB11B, Ras related protein Rab 11B, YPT3



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, fetal human brain tissue, human testis tissue, PC-12 cells, HEK-293 cells, mouse testis tissue, C6 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung tissue, human brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

19742-1-AP targets RAB11B-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB11B, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_004218
Gene symbol RAB11B
Gene ID (NCBI) 9230
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.

Protocols

Product Specific Protocols
WB protocol for RAB11B-Specific antibody 19742-1-APDownload protocol
IHC protocol for RAB11B-Specific antibody 19742-1-APDownload protocol
IP protocol for RAB11B-Specific antibody 19742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Neurosci

The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.

Authors - Wataru Otsu
canine,ratWB,IHC

J Histochem Cytochem

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

Authors - Hiroshi Gomi
humanWB

Tumour Biol

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Authors - Zhaoyang Zeng
humanWB

J Exp Clin Cancer Res

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

Authors - Yu Lian
humanWB

Oncotarget

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Authors - Hao Bo