|Positive WB detected in||HeLa cells, SGC-7901 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue|
|Positive IP detected in||SGC-7901 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11091-1-AP targets EMAP II in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EMAP II fusion protein Ag1560|
|Full Name||small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa and 68 kDa|
|GenBank accession number||BC014051|
|Gene ID (NCBI)||9255|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).
|Product Specific Protocols|
|WB protocol for EMAP II antibody 11091-1-AP||Download protocol|
|IHC protocol for EMAP II antibody 11091-1-AP||Download protocol|
|IF protocol for EMAP II antibody 11091-1-AP||Download protocol|
|IP protocol for EMAP II antibody 11091-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.
J Biol Chem
Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Krishnendu (Verified Customer) (02-27-2020)
Very strong antibody and works nicely for both WB and IP.