EMAP II Polyclonal antibody

EMAP II Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11091-1-AP

Synonyms

AIMP1, EMAP 2, EMAP II, EMAP2, EMAPII, p43, SCYE1



Tested Applications

Positive WB detected inHeLa cells, SGC-7901 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue
Positive IP detected inSGC-7901 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

11091-1-AP targets EMAP II in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EMAP II fusion protein Ag1560
Full Name small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa and 68 kDa
GenBank accession numberBC014051
Gene symbol SCYE1
Gene ID (NCBI) 9255
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).

Protocols

Product Specific Protocols
WB protocol for EMAP II antibody 11091-1-APDownload protocol
IHC protocol for EMAP II antibody 11091-1-APDownload protocol
IF protocol for EMAP II antibody 11091-1-APDownload protocol
IP protocol for EMAP II antibody 11091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.

Authors - Wei Gao
humanWB

J Biol Chem

Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains.

Authors - Dalia Halawani

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Krishnendu (Verified Customer) (02-27-2020)

Very strong antibody and works nicely for both WB and IP.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 for WB and 1:100 for IP
  • Cell Tissue Type: HEK293T