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  • Western Blot (WB) analysis of various lysates using SDS Polyclonal antibody (16594-1-AP)

    WB analysis using 16594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SDS Polyclonal antibody (16594-1-AP)

    WB analysis using 16594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SDS Polyclonal antibody (16594-1-AP)

    IHC staining of human liver using 16594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SDS Polyclonal antibody (16594-1-AP)

    IHC staining of human liver using 16594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SDS Polyclonal antibody

SDS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16594-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:4.3.1.17, EC:4.3.1.19, L serine deaminase, L threonine dehydratase, L-serine deaminase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16594-1-AP targets SDS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDS fusion protein Ag9704 Predict reactive species
Full Name serine dehydratase
Calculated Molecular Weight 218 aa, 23 kDa
Observed Molecular Weight35 kDa; 70 kDa
GenBank Accession NumberBC020750
Gene Symbol SDS
Gene ID (NCBI) 10993
RRIDAB_3085498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L-Serine dehydratase (SDS ; SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of l-serine to yield pyruvate. In mammals, SDH is found predominantly in the liver. Extensive studies have been carried out on SDH from rat and the enzyme has been found to play an important role in gluconeogenesis; its activity is induced by the consumption of high-protein diets, starvation and other treatments. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. (PMID: 25380533, PMID: 21878319, PMID: 14646100)

Protocols

Product Specific Protocols
WB protocol for SDS antibody 16594-1-APDownload protocol
IHC protocol for SDS antibody 16594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols