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SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11B4

Cat no : 60281-1-Ig

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1

Validation Data Gallery

  • WB analysis of PC-3 using 60281-1-Ig

    WB analysis of PC-3 using 60281-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 60281-1-Ig

    IHC staining of human colon cancer using 60281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60281-1-Ig (SECTM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 60281-1-Ig

    IHC staining of human colon cancer using 60281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60281-1-Ig (SECTM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 60281-1-Ig

    FC experiment of K-562 using 60281-1-Ig

    1X10^6 K-562 cells were stained with 0.2ug SECTM1 antibody (60281-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inPC-3 cells, K-562 cells, Transfected HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60281-1-Ig targets SECTM1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SECTM1 fusion protein Ag14645
Full Name secreted and transmembrane 1
Calculated molecular weight 248 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC017716
Gene symbol SECTM1
Gene ID (NCBI) 6398
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.

Protocols

Product Specific Protocols
WB protocol for SECTM1 antibody 60281-1-IgDownload protocol
IHC protocol for SECTM1 antibody 60281-1-IgDownload protocol
FC protocol for SECTM1 antibody 60281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols