SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11B4

Cat no : 60281-1-Ig

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1



Tested Applications

Positive WB detected inPC-3 cells, K-562 cells, Transfected HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60281-1-Ig targets SECTM1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SECTM1 fusion protein Ag14645
Full Name secreted and transmembrane 1
Calculated molecular weight 248 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC017716
Gene symbol SECTM1
Gene ID (NCBI) 6398
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.

Protocols

Product Specific Protocols
WB protocol for SECTM1 antibody 60281-1-IgDownload protocol
IHC protocol for SECTM1 antibody 60281-1-IgDownload protocol
FC protocol for SECTM1 antibody 60281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols