Tested Applications
| Positive WB detected in | 37°C incubated mouse heart tissue, 37℃ incubated mouse heart, rat heart, 37℃ incubated rat heart |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86050-1-RR targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag26177 Product name: Recombinant human SERCA2,ATP2A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 314-580 aa of BC035588 Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDS Predict reactive species |
| Full Name | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| Calculated Molecular Weight | 1042 aa, 115 kDa |
| Observed Molecular Weight | 110-115 kDa |
| GenBank Accession Number | BC035588 |
| Gene Symbol | SERCA2 |
| Gene ID (NCBI) | 488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16615 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SERCA2/ATP2A2 antibody 86050-1-RR | Download protocol |
| IHC protocol for SERCA2/ATP2A2 antibody 86050-1-RR | Download protocol |
| WB protocol for SERCA2/ATP2A2 antibody 86050-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
