SERCA2/ATP2A2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86050-1-RR (same clone as 86050-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86050-1-RR (SERCA2,ATP2A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86050-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 86050-1-RR (same clone as 86050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86050-1-RR (SERCA2/ATP2A2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86050-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 86050-1-RR (same clone as 86050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody (86050-1-RR, Clone: 250667D7 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86050-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86050-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86050-1-RR against Human SERCA2/ATP2A2 were performed. The affinity constant is below 1 pM.

SERCA2/ATP2A2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86050-1-PBS

Clone No.250667D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP2A2, SERCA2, SERCA2b, ATP2A 2, ATP2B

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Product Information

86050-1-PBS targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26177

  Product name: Recombinant human SERCA2,ATP2A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 314-580 aa of BC035588

  Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDS

• Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calculated Molecular Weight: 1042 aa, 115 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC035588
• Gene Symbol: SERCA2
• Gene ID (NCBI): 488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16615
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.