14-3-3 Sigma Monoclonal antibody

14-3-3 Sigma Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F10D9

Cat no : 66251-1-Ig

Synonyms

14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS



Tested Applications

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:8000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66251-1-Ig targets 14-3-3 Sigma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 14-3-3 Sigma fusion protein Ag12644
Full Name stratifin
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC002995
Gene symbol SFN
Gene ID (NCBI) 2810
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
IHC protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
IF protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi