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  • Featured Product
  • KD/KO Validated

SLC25A6-Specific Polyclonal antibody

SLC25A6-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14841-1-AP

Synonyms

AAC3, ADP,ATP carrier protein 3, ADP/ATP translocase 3, ANT 2, ANT 3, ANT3, ANT3Y, SLC25A6, SLC25A6-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    WB analysis using 14841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    WB analysis of HEK-293 using 14841-1-AP

    WB result of SLC25A6-Specific antibody (14841-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A6-Specific transfected HEK-293 cells.

  • Western Blot (WB) analysis of human liver tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    WB analysis of human liver using 14841-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    WB analysis of human heart using 14841-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    WB analysis of HEK-293 using 14841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IP experiment of mouse liver using 14841-1-AP

    IP result of anti-SLC25A6-Specific (IP:14841-1-AP, 4ug; Detection:14841-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human skeletal muscle using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human skeletal muscle using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human kidney using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human kidney using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human liver using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human liver using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human heart using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IHC staining of human heart using 14841-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IF Staining of HepG2 using 14841-1-AP

    Immunofluorescent analysis of HepG2 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SLC25A6-Specific Polyclonal antibody (14841-1-AP)

    IF Staining of MCF-7 using 14841-1-AP

    Immunofluorescent analysis of MCF-7 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells, human liver tissue, human heart tissue, human placenta tissue, HEK-293T cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14841-1-AP targets SLC25A6-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-33 kDa
GenBank Accession NumberNM_001636
Gene Symbol SLC25A6
Gene ID (NCBI) 293
RRIDAB_2190371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC25A6-Specific antibody 14841-1-APDownload protocol
IHC protocol for SLC25A6-Specific antibody 14841-1-APDownload protocol
IF protocol for SLC25A6-Specific antibody 14841-1-APDownload protocol
IP protocol for SLC25A6-Specific antibody 14841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Biol

Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.

Authors - Ridhima Gomkale
View Article
WB

Hum Mol Genet

Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.

Authors - David Pacheu-Grau
View Article
humanWB,IF

Cancer Cell Int

Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.

Authors - Yi Hao
View Article
humanWB

FEBS Lett

TIM29 is a subunit of the human carrier translocase required for protein transport.

Authors - Sylvie Callegari
View Article
humanWB,IHC,CoIP

iScience

MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells

Authors - Xingzhao Ji
View Article