Tested Applications
| Positive WB detected in | L02 cells, HEK-293 cells, human liver tissue, human heart tissue, human placenta tissue, HEK-293T cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14841-1-AP targets SLC25A6-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | NM_001636 |
| Gene Symbol | SLC25A6 |
| Gene ID (NCBI) | 293 |
| RRID | AB_2190371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| IHC protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| IP protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| WB protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SLC25A6 (ANT3) antibody (Cat# 14841-1-AP) has 10 total citations spanning journals including J Clin Invest, Adv Sci, Cell Death Dis, Curr Biol, Cancer Cell Int, ACS Omega, iScience, Hum Mol Genet, FEBS Lett, and Am J Cancer Res. Research fields cover mitochondrial biology, ferroptosis, cancer (ovarian, cervical, pancreatic), acute kidney injury, inflammation, mitochondrial myopathy, and protein transport across the inner mitochondrial membrane.
| Species | Application | Title |
|---|---|---|
J Clin Invest Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cells | ||
Adv Sci (Weinh) CypD Dependent mPTP Opening Is Crucial for Oxidized Mitochondrial DNA Release in Ferroptosis. | ||
Cell Death Dis MRPL13 enhances mitochondrial function and promotes tumor progression in ovarian cancer by inhibiting mPTP opening via SLC25A6 | ||
Curr Biol Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos. | ||
Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer. | ||
ACS Omega Carvacrol Regulates the Expression of SLC25A6 by Inhibiting VDAC1 to Improve Mitochondrial Function and Reduce LPS-Induced Inflammatory Injury in HMEC-1 Cells |
