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SLK Polyclonal antibody

SLK Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19743-1-AP

Synonyms

CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLK Polyclonal antibody (19743-1-AP)

    WB analysis using 19743-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SLK Polyclonal antibody (19743-1-AP)

    WB analysis of human brain using 19743-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SLK Polyclonal antibody (19743-1-AP)

    WB analysis of MCF-7 using 19743-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SLK Polyclonal antibody (19743-1-AP)

    WB analysis of mouse liver using 19743-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLK Polyclonal antibody (19743-1-AP)

    IF Staining of HepG2 using 19743-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLK antibody (19743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, MCF-7 cells, mouse liver tissue, HeLa cells, THP-1 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19743-1-AP targets SLK in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name STE20-like kinase (yeast)
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 143-160 kDa, 210-220 kDa
GenBank Accession NumberNM_014720
Gene Symbol SLK
Gene ID (NCBI) 9748
RRIDAB_10644278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)

Protocols

Product Specific Protocols
WB protocol for SLK antibody 19743-1-APDownload protocol
IF protocol for SLK antibody 19743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols