CoraLite® Plus 488-conjugated SLK Polyclonal antibody

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  IF Staining of HepG2 using CL488-19743

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SLK antibody (CL488-19743) at dilution of 1:200, CL594-Phalloidin (red).

SLK Polyclonal Antibody for IF/ICC

Cat No. CL488-19743

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

bA16H23.1, CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19743 targets SLK in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: STE20-like kinase (yeast)
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 143-160 kDa, 210-220 kDa
• GenBank Accession Number: NM_014720
• Gene Symbol: SLK
• Gene ID (NCBI): 9748
• RRID: AB_3672708
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2G2
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)