SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 18839-1-AP

Synonyms

hSorCS, SORCS1



Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, mouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18839-1-AP targets SORCS1 in WB, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sortilin-related VPS10 domain containing receptor 1
Calculated Molecular Weight 1168 aa, 130 kDa
Observed Molecular Weight 130 kDa, 60-80 kDa
GenBank Accession NumberBC131597
Gene Symbol SORCS1
Gene ID (NCBI) 114815
RRIDAB_2878564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SorCS1 is a 130 kDa type I transmembrane protein, belonging to Vps10p-domain (Vps10p-D) receptor family that comprises an N-terminal Vps10p-D, a leucine-rich domain, a single transmembrane domain, and a short cytoplasmic domain. SorCS1 is synthesized as a proprotein, which is converted to the mature receptor in late Golgi compartments by cleavage of the N-terminal propeptide. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic domains and differential expression in tissues. This antibody was generated against the C-terminal region of human SorCS1 and recognizes various forms of SorCS1. Multiple bands around 100-130 kDa as well as 60-80 kDa have been detected in Y79 cells by western blot assay.

Protocols

Product Specific Protocols
WB protocol for SORCS1 antibody 18839-1-APDownload protocol
FC protocol for SORCS1 antibody 18839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols