Tested Applications
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16725-1-AP targets SOX15 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10173 Product name: Recombinant human SOX15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC000985 Sequence: MALPGSSQDQAWSLEPPAATAAASSSSGPQEREGAGSPAAPGTLPLEKVKRPMNAFMVWSSAQRRQMAQQNPKMHNSEISKRLGAQWKLLDEDEKRPFVEEAKRLRARHLRDYPDYKYRPRRKAKSSGAGPSRCGQGRGNLASGGPLWGPGYATTQPSRGFGYRPPSYSTAYLPGSYGSSHCKLEAPSPCSLPQSDPRLQGELLPTYTHYLPPGSPTPYNPPLAGAPMPLTHL Predict reactive species |
| Full Name | SRY (sex determining region Y)-box 15 |
| Calculated Molecular Weight | 25 kDa |
| GenBank Accession Number | BC000985 |
| Gene Symbol | SOX15 |
| Gene ID (NCBI) | 6665 |
| RRID | AB_2878305 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60248 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SOX15 antibody 16725-1-AP | Download protocol |
| IHC protocol for SOX15 antibody 16725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SOX15 Polyclonal antibody (Cat# 16725-1-AP) has 1 total citation. It appears in Cell Reports (IF 7.7), published in November 2022. The cited study investigated single-cell transcriptome and chromatin accessibility during early gonad development across goats, pigs, macaques, and humans. The antibody was applied in immunofluorescence (IF) with human and pig samples, placing its research field in developmental biology and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cell Rep Integration of single-cell transcriptome and chromatin accessibility of early gonads development among goats, pigs, macaques, and humans |
