SREBF2 Polyclonal antibody

SREBF2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 14508-1-AP

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2



Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, HepG2 cells, human kidney tissue, HEK-293 cells, rat kidney tissue, HeLa cells, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14508-1-AP targets SREBF2 in WB, IP, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SREBF2 fusion protein Ag5967
Full Name sterol regulatory element binding transcription factor 2
Calculated Molecular Weight 124 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC056158
Gene Symbol SREBF2
Gene ID (NCBI) 6721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.

Protocols

Product Specific Protocols
WB protocol for SREBF2 antibody 14508-1-APDownload protocol
IHC protocol for SREBF2 antibody 14508-1-APDownload protocol
IP protocol for SREBF2 antibody 14508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.

Authors - Jinbo Han
mouseWB

Nature

TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.

Authors - Joanne Hsieh
mouseWB

Environ Pollut

Prenatal exposure to diesel exhaust PM2.5 programmed non-alcoholic fatty liver disease differently in adult male offspring of mice fed normal chow and a high-fat diet.

Authors - Xiaoke Wang
human, mouseWB

Arterioscler Thromb Vasc Biol

RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.

Authors - Yan-Wei Hu
mouse, ratWB

Arch Toxicol

Protective effects of dioscin against alcohol-induced liver injury.

Authors - Xu Tingting T
mouseIHC

J Lipid Res

Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein.

Authors - Ya-Qi Wang