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STAT3 Polyclonal antibody

STAT3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10253-2-AP

Synonyms

Acute phase response factor, APRF, HIES, STAT3



Tested Applications

Positive WB detected inA549 cells, mouse lung tissue, Jurkat cells, A431 cells, mouse brain tissue, K-562 cells, mouse heart tissue, MCF-7 cells, NIH/3T3 cells, rat brain tissue, HeLa cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human breast cancer tissue, human cervical cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10253-2-AP targets STAT3 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, marmoset, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAT3 fusion protein Ag0360
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated molecular weight 770 aa, 88 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC000627
Gene symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Protocols

Product Specific Protocols
WB protocol for STAT3 antibody 10253-2-APDownload protocol
IHC protocol for STAT3 antibody 10253-2-APDownload protocol
IF protocol for STAT3 antibody 10253-2-APDownload protocol
IP protocol for STAT3 antibody 10253-2-APDownload protocol
FC protocol for STAT3 antibody 10253-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Med Sci Monit

The Adenosine Monophosphate (AMP) Analog, 5-Aminoimidazole-4-Carboxamide Ribonucleotide (AICAR) Inhibits Hepatosteatosis and Liver Tumorigenesis in a High-Fat Diet Murine Model Treated with Diethylnitrosamine (DEN).

Authors - Jianlong Gao
humanCoIP

Int J Mol Sci

IL-8 Secreted from M2 Macrophages Promoted Prostate Tumorigenesis via STAT3/MALAT1 Pathway.

Authors - Tingjin Zheng
humanWB

J Immunol

IL-27 Promotes Human Placenta-Derived Mesenchymal Stromal Cell Ability To Induce the Generation of CD4+IL-10+IFN-γ+ T Cells via the JAK/STAT Pathway in the Treatment of Experimental Graft-versus-Host Disease.

Authors - Junzhu Yi
humanWB

Sci Rep

Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.

Authors - Zhaoxing Li
humanWB

Cancer Res

Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer.

Authors - Dalong Cao
humanWB

J Pharmacol Sci

Cucurbitacin B induces inhibitory effects via the CIP2A/PP2A/C-KIT signaling axis in t(8;21) acute myeloid leukemia.

Authors - Wenjing Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (01-23-2020)

Very good Stat3 antibody for western blot. Strong signal at 1:5000 dilution. Highly recommend.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Hela
FH

Daniele (Verified Customer) (11-25-2019)

It works very well both in western blot and in immunoprecipitation.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000 (western blot)
  • Cell Tissue Type: MDA MB 468 and A375