STEAP2-Specific Antibody

STEAP2-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 20201-1-AP

Synonyms

IPCA1, Metalloreductase STEAP2, PCANAP1, PUMPCn, STAMP1, STEAP2, STMP



Tested Applications

Positive WB detected inHEK-293 cells, DU 145 cells, fetal human brain tissue, HeLa cells, human brain tissue, mouse kidney tissue
Positive IHC detected inhuman heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

20201-1-AP targets STEAP2-Specific in IHC, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name six transmembrane epithelial antigen of the prostate 2
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession number NM_152999
Gene symbol STEAP2
Gene ID (NCBI) 261729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

Protocols

Product Specific Protocols
WB protocol for STEAP2-Specific antibody 20201-1-APDownload protocol
IHC protocol for STEAP2-Specific antibody 20201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Cell Res

Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution.

Authors - Haruki Hasegawa