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SUB1 Polyclonal antibody

SUB1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10948-2-AP

Synonyms

p14, PC4, Positive cofactor 4, RPO2TC1, SUB1, SUB1 homolog, SUB1 homolog (S. cerevisiae)



Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10948-2-AP targets SUB1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUB1 fusion protein Ag1390
Full Name SUB1 homolog (S. cerevisiae)
Calculated molecular weight 14 kDa
Observed molecular weight 18-23 kDa
GenBank accession numberBC009610
Gene symbol SUB1
Gene ID (NCBI) 10923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#10948-2-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).

Protocols

Product Specific Protocols
WB protocol for SUB1 antibody 10948-2-APDownload protocol
IHC protocol for SUB1 antibody 10948-2-APDownload protocol
IF protocol for SUB1 antibody 10948-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biochem

Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes.

Authors - Li Lu L
mouseWB,IHC

J Proteome Res

Novel breast cancer metastasis-associated proteins.

Authors - Ho Jiapei J