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  • Featured Product
  • KD/KO Validated

SUB1 Polyclonal antibody

SUB1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10948-2-AP

Synonyms

p14, PC4, Positive cofactor 4, RPO2TC1, SUB1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis of A549 using 10948-2-AP

    WB result of SUB1 antibody (10948-2-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis of HeLa using 10948-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis of HeLa using 10948-2-AP

    WB result of SUB1 antibody (10948-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis using 10948-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis of HeLa using 10948-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis of A549 using 10948-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SUB1 Polyclonal antibody (10948-2-AP)

    WB analysis of A549 using 10948-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SUB1 Polyclonal antibody (10948-2-AP)

    IHC staining of human pancreas cancer using 10948-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SUB1 Polyclonal antibody (10948-2-AP)

    IHC staining of human pancreas cancer using 10948-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SUB1 Polyclonal antibody (10948-2-AP)

    IHC staining of human pancreas cancer using 10948-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SUB1 Polyclonal antibody (10948-2-AP)

    IHC staining of human pancreas cancer using 10948-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUB1 Polyclonal antibody (10948-2-AP)

    IF Staining of HeLa using 10948-2-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10948-2-AP (SUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10948-2-AP targets SUB1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUB1 fusion protein Ag1390
Full Name SUB1 homolog (S. cerevisiae)
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC009610
Gene Symbol SUB1
Gene ID (NCBI) 10923
RRIDAB_2255452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#10948-2-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).

Protocols

Product Specific Protocols
WB protocol for SUB1 antibody 10948-2-APDownload protocol
IHC protocol for SUB1 antibody 10948-2-APDownload protocol
IF protocol for SUB1 antibody 10948-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

J Proteome Res

Novel breast cancer metastasis-associated proteins.

Authors - Ho Jiapei J
View Article
mouseWB

J Cell Biochem

Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes.

Authors - Li Lu L
View Article
human,mouseWB,IHC,IF,CoIP

Sci China Life Sci

SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1

Authors - Hao Wang
  • KO Validated
View Article