Synaptophysin Antibody
Synaptophysin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
Cat no : 17785-1-AP
Synonyms
SYN, synaptophysin, SYP
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human pituitary adenoma tissue, ganglioma tissue, neuroblastoma tissue, human brain tissue, human pancreas tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-12 cells, human lung cancer tissue, human gliomas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 26 publications below |
| IHC | See 2 publications below |
| IF | See 13 publications below |
Product Information
17785-1-AP targets Synaptophysin in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Reactivity | human, mouse, rat |
| Cited Species | hamster, hamsters, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synaptophysin fusion protein Ag11781 |
| Full Name | synaptophysin |
| Calculated molecular weight | 313aa,34 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | BC064550 |
| Gene symbol | SYP |
| Gene ID (NCBI) | 6855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20oC. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| IHC protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| IF protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| IP protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Toxicol Cadmium induces actin cytoskeleton alterations and dysfunction in Neuro-2a cells. | ||
Cell A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. | ||
Front Psychiatry Brain-Derived Neurotrophic Factor Precursor in the Hippocampus Regulates Both Depressive and Anxiety-Like Behaviors in Rats. | ||
Int J Mol Sci Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway. | ||
Cells MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model. |
