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Synaptophysin Antibody 22 Publications

Rabbit Polyclonal| Catalog number: 17785-1-AP

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Synaptophysin Antibody WB mouse brain tissue 17785-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours

Synaptophysin Antibody IHC human pancreas tissue 17785-1-AP

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 17785-1-AP( Synaptophysin antibody at dilution of 1:200 (under 10x lens).

Synaptophysin Antibody IHC human pancreas tissue 17785-1-AP

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 17785-1-AP( Synaptophysin antibody at dilution of 1:200 (under 40x lens).

Synaptophysin Antibody IF PC-12 cells 17785-1-AP

Immunofluorescent analysis of PC-12 cells using 17785-1-AP(Synaptophysin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Synaptophysin Antibody IF human lung cancer tissue 17785-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Synaptophysin Antibody IP mouse brain tissue 17785-1-AP

IP Result of anti-Synaptophysin; SYP (IP:17785-1-AP, 3ug; Detection:17785-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Species specificity:
human, mouse, rat, hamster
Positive WB detected in:
mouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human pancreas tissue, human pituitary adenoma tissue, ganglioma tissue, mouse pancreas tissue, neuroblastoma tissue, human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
PC-12 cells, human lung cancer tissue, human gliomas tissue
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Synaptophysin fusion protein Ag11781
Full name:
synaptophysin
Calculated molecular weight:
313aa,34 kDa
Observed molecular weight:
38-40 kDa
GenBank accession number:
BC064550
Gene ID (NCBI):
6855
Gene symbol
SYP
Synonyms
SYN, synaptophysin, SYP

Background

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)


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