Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 17785-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.
WB analysis using 17785-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis using 17785-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17785-1-AP
IP result of anti-Synaptophysin (IP:17785-1-AP, 3ug; Detection:17785-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of mouse brain using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of ganglioma using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded ganglioma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).
IHC staining of ganglioma using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded ganglioma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).
IHC staining of neuroblastoma using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).
IHC staining of neuroblastoma using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 40x lens).
IHC staining of human pituitary adenoma using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).
IHC staining of human pituitary adenoma using 17785-1-AP
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).
IF Staining of mouse pancreas using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse pancreas using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human lung cancer using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human lung cancer using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human gliomas using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human gliomas using 17785-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of PC-12 using 17785-1-AP
Immunofluorescent analysis of PC-12 cells using 17785-1-AP (Synaptophysin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Synaptophysin Polyclonal antibody

Name: Synaptophysin Polyclonal antibody
Brand: Proteintech
SKU: 17785-1-AP
Rating: 5.00 (2 reviews)

Synaptophysin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 17785-1-AP

Publications(238)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA, IHC-IF

Synonyms

SYP, MRX96, SYN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (238)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, PC-12 cells, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse pancreas tissue, mouse brain tissue, human brain tissue, ganglioma tissue, human pancreas tissue, neuroblastoma tissue, human pituitary adenoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse pancreas tissue, human lung cancer tissue, human gliomas tissue, mouse brain tissue
Positive IF/ICC detected in	PC-12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 185 publications below
IHC	See 24 publications below
IF	See 57 publications below

Product Information

17785-1-AP targets Synaptophysin in WB, IHC, IF/ICC, IF-P, IP, ELISA, IHC-IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synaptophysin fusion protein Ag11781 Predict reactive species
Full Name	synaptophysin
Calculated Molecular Weight	313 aa, 34 kDa
Observed Molecular Weight	38-40 kDa
GenBank Accession Number	BC064550
Gene Symbol	Synaptophysin
Gene ID (NCBI)	6855
RRID	AB_2271365
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08247
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for the identification of neuroendocrine cells and neoplasms. Synaptic proteins including synaptophysin are reduced in brain tissue from patients with Alzheimer's disease.(PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
WB protocol for Synaptophysin antibody 17785-1-AP	Download protocol
IHC protocol for Synaptophysin antibody 17785-1-AP	Download protocol
IF protocol for Synaptophysin antibody 17785-1-AP	Download protocol
IP protocol for Synaptophysin antibody 17785-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy Authors - Aleksandra Klimas View Article
rat,mouse	WB,IF	Nat Genet Identification of TMEM230 mutations in familial Parkinson's disease. Authors - Han-Xiang Deng View Article
mouse	WB,IF	Nat Neurosci Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage Authors - Yao Wei View Article
mouse	IF	Mol Cell Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury. Authors - Zhengxin Ying View Article
mouse	WB	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
mouse	IF	Neuron Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2. Authors - Nathalie Escande-Beillard View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Alexandra (Verified Customer) (06-11-2023)

Hoechst-blue Green-Tubulin III (neuronal marker) Orange (Synaptophysin)

Applications: Immunofluorescence
Primary Antibody Dilution: 1:300
Cell Tissue Type: P19 derived neurons

Synaptophysin Antibody Immunofluorescence validation (1:300 dilution) in P19 derived neurons (Cat no:17785-1-AP)

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)