Validation Data Gallery
|Positive WB detected in||mouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue, human pituitary adenoma tissue, ganglioma tissue, neuroblastoma tissue, human brain tissue, human pancreas tissue, mouse pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells, human lung cancer tissue, human gliomas tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17785-1-AP targets Synaptophysin in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||hamster, hamsters, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synaptophysin fusion protein Ag11781|
|Calculated molecular weight||313aa,34 kDa|
|Observed molecular weight||38-40 kDa|
|GenBank accession number||BC064550|
|Gene ID (NCBI)||6855|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
|Product Specific Protocols|
|WB protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|IHC protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|IF protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|IP protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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