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TDP-43 Polyclonal antibody
TDP-43 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 10782-2-AP
Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells|
|Positive IHC detected in||human gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10782-2-AP targets TDP-43 in WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, kak samples.
|Tested Reactivity||human, mouse, rat, zebrafish, kak|
|Cited Reactivity||human, mouse, Caenorhabditis elegans, canine, chicken, Drosophila, hamster, horse, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Mutant TDP-43 Causes Early-Stage Dose-Dependent Motor Neuron Degeneration in a TARDBP Knockin Mouse Model of ALS.
TDP-43 levels are higher in platelets from patients with sporadic amyotrophic lateral sclerosis than in healthy controls.
Acta Neuropathol Commun
Drosophila Ref1/ALYREF regulates transcription and toxicity associated with ALS/FTD disease etiologies.
ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair.
ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.
Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Pauline (Verified Customer) (02-23-2022)
Xin (Verified Customer) (01-23-2022)
Very good in WB with a band of around 45 kD.
Jessica (Verified Customer) (06-10-2021)
good for WB and IF
Ana (Verified Customer) (03-14-2021)
TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.
Yasuyo (Verified Customer) (01-20-2021)
Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.
MEIMEI (Verified Customer) (11-12-2020)
the antibody work prefect for my staining.
Stephane (Verified Customer) (10-31-2020)
Good product for staining.
Marina (Verified Customer) (10-25-2020)
TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.
Uxoa (Verified Customer) (02-18-2020)
Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.
Paul (Verified Customer) (01-15-2020)
Produces good IF images with minimal background.
Laura (Verified Customer) (01-10-2020)
Works very well
Benjamin (Verified Customer) (01-07-2020)
Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.
Apoorva (Verified Customer) (12-18-2019)
It has great specificity over wide range of species.
Tilly (Verified Customer) (11-27-2019)
Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.
Azita (Verified Customer) (10-04-2019)
I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.
Francisco (Verified Customer) (09-28-2019)
Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.
Alinda (Verified Customer) (09-11-2019)
Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.
David (Verified Customer) (07-18-2019)
Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.
George (Verified Customer) (07-07-2019)
Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.
Elena (Verified Customer) (08-17-2018)
Petra (Verified Customer) (03-06-2018)