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  • KD/KO Validated

TDP-43 Antibody

TDP-43 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (8)

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 10782-2-AP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43



Tested Applications

Positive WB detected inSH-SY5Y cells, K-562 cells, HeLa cells, C2C12 cells, Neuro-2a cells
Positive IHC detected inmouse brain tissue, human brain tissue, human brain (FTLD-U) tissue, human gliomas tissue, human pancreas tissue, rat brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells, SH-SY5Y cells, HeLa cells, mouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10782-2-AP targets TDP-43 in FC, IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Reactivity human, mouse, rat, zebrafish
Cited Species C. elegans, chicken, dog, Drosophila, fly, hamster, horse, human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession number BC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.

In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

Proteintech’s 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 10782-2-APDownload protocol
IHC protocol for TDP-43 antibody 10782-2-APDownload protocol
IF protocol for TDP-43 antibody 10782-2-APDownload protocol
FC protocol for TDP-43 antibody 10782-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Acta Neuropathol Commun

Dichotomous scoring of TDP-43 proteinopathy from specific brain regions in 27 academic research centers: associations with Alzheimer's disease and cerebrovascular disease pathologies.

Authors - Yuriko Katsumata
humanWB

Neurochem Int

TDP-43 levels are higher in platelets from patients with sporadic amyotrophic lateral sclerosis than in healthy controls.

Authors - Miki Hishizawa
WB

Structure

Structure of Calcarisporiella thermophila Hsp104 Disaggregase that Antagonizes Diverse Proteotoxic Misfolding Events.

Authors - Karolina Michalska
mouseWB,IF

Cell Rep

Mutant TDP-43 Causes Early-Stage Dose-Dependent Motor Neuron Degeneration in a TARDBP Knockin Mouse Model of ALS.

Authors - Sarah Y Ebstein
humanWB,IF

J Biol Chem

Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.

Authors - Youjun Chen
humanWB,IF

Nat Neurosci

ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair.

Authors - Joseph R Klim
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Uxoa (Verified Customer) (02-18-2020)

Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human fibroblasts
TDP-43 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:10782-2-AP)
FH

Paul (Verified Customer) (01-15-2020)

Produces good IF images with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HEK293T
No image submitted with review
FH

Laura (Verified Customer) (01-10-2020)

Works very well

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
No image submitted with review
FH

Benjamin (Verified Customer) (01-07-2020)

Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:2500, 1:100
  • Cell Tissue Type: HEK293T
No image submitted with review
FH

Apoorva (Verified Customer) (12-18-2019)

It has great specificity over wide range of species.

  • Applications: Western Blot,
No image submitted with review
FH

Tilly (Verified Customer) (11-27-2019)

Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: IHC and WB: 1:1000. IF: 1:400
  • Cell Tissue Type: Mouse brain tissue
No image submitted with review
FH

Azita (Verified Customer) (10-04-2019)

I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/500-1/300-1/100- 1/50
  • Cell Tissue Type: NSC-34- Primary Hippocampal Neuron Culture (Rat P0-P1 Pups)
No image submitted with review
FH

Francisco (Verified Customer) (09-28-2019)

Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse
No image submitted with review
FH

Alinda (Verified Customer) (09-11-2019)

Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SHSY5Y
TDP-43 Antibody Western Blot, validation (1:1000 dilution) in SHSY5Y (Cat no:10782-2-AP)
FH

David (Verified Customer) (07-18-2019)

Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (IB), 1:500 (ICC)
  • Cell Tissue Type: Neuroblastoma, iPSC-derived neurons
No image submitted with review
FH

George (Verified Customer) (07-07-2019)

Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Spinal Cord/Brain slices
No image submitted with review
FH

Elena (Verified Customer) (08-17-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK-293 cells
No image submitted with review
FH

Petra (Verified Customer) (03-06-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue
No image submitted with review