TDP-43 Antibody 829 Publications

Rabbit Polyclonal| Catalog number: 10782-2-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 46 μg/150 μl

Choose size:

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Tested Applications

Applications:
ELISA, ChIP, CoIP, FC, IF, IHC, Immunoelectron, IP, RIP, WB
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Species specificity:
human, mouse, rat, zebrafish, C. elegans, chicken, dog, Drosophila, fly, hamster, horse, monkey, recombinant protein, worm, Yeast

Positive WB detected in:
A549 cells, mouse brain tissue, human heart tissue, K-562 cells, HL-60 cells, mouse pancreas tissue, HeLa cells, rat brain tissue

Positive IP detected in:
HeLa cells, K-562 cells

Positive IHC detected in:
human brain (FTLD-U) tissue, human gliomas tissue

Positive IF detected in:
HeLa cells, mouse brain tissue, HepG2 cells, MCF-7 cells, SH-SY5Y cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:5000
IF : 1:200-1:800

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
TAR DNA binding protein

Calculated molecular weight:
43 kDa

Observed molecular weight:
43 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TARDBP

Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Background

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.

In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

Proteintech’s 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. Generated using the first 260 amino acids of TDP-43, it recognizes the intact 45 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)


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