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TDP-43 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 10782-2-AP
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, K-562 cells, HeLa cells, C2C12 cells, Neuro-2a cells|
|Positive IHC detected in||mouse brain tissue, human brain tissue, human brain (FTLD-U) tissue, human gliomas tissue, human pancreas tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, MCF-7 cells, SH-SY5Y cells, HeLa cells, mouse brain tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10782-2-AP targets TDP-43 in FC, IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
|Reactivity||human, mouse, rat, zebrafish|
|Cited Species||C. elegans, chicken, dog, Drosophila, fly, hamster, horse, human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.
In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
Proteintech’s 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
|Product Specific Protocols|
|WB protocol for TDP-43 antibody 10782-2-AP||Download protocol|
|IHC protocol for TDP-43 antibody 10782-2-AP||Download protocol|
|IF protocol for TDP-43 antibody 10782-2-AP||Download protocol|
|FC protocol for TDP-43 antibody 10782-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Acta Neuropathol Commun
Dichotomous scoring of TDP-43 proteinopathy from specific brain regions in 27 academic research centers: associations with Alzheimer's disease and cerebrovascular disease pathologies.
TDP-43 levels are higher in platelets from patients with sporadic amyotrophic lateral sclerosis than in healthy controls.
Structure of Calcarisporiella thermophila Hsp104 Disaggregase that Antagonizes Diverse Proteotoxic Misfolding Events.
Mutant TDP-43 Causes Early-Stage Dose-Dependent Motor Neuron Degeneration in a TARDBP Knockin Mouse Model of ALS.
J Biol Chem
Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.
ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Uxoa (Verified Customer) (02-18-2020)
Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.
Paul (Verified Customer) (01-15-2020)
Produces good IF images with minimal background.
Laura (Verified Customer) (01-10-2020)
Works very well
Benjamin (Verified Customer) (01-07-2020)
Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.
Apoorva (Verified Customer) (12-18-2019)
It has great specificity over wide range of species.
Tilly (Verified Customer) (11-27-2019)
Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.
Azita (Verified Customer) (10-04-2019)
I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.
Francisco (Verified Customer) (09-28-2019)
Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.
Alinda (Verified Customer) (09-11-2019)
Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.
David (Verified Customer) (07-18-2019)
Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.
George (Verified Customer) (07-07-2019)
Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.
Elena (Verified Customer) (08-17-2018)
Petra (Verified Customer) (03-06-2018)