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TH Monoclonal antibody

TH Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H7B7

Cat no : 66334-1-Ig

Synonyms

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase



Tested Applications

Positive WB detected inPC-12 cells, SH-SY5Y cells
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66334-1-Ig targets TH in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TH fusion protein Ag23075
Full Name tyrosine hydroxylase
Calculated molecular weight 528 aa, 59 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC104967
Gene symbol TH
Gene ID (NCBI) 7054
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.

Protocols

Product Specific Protocols
WB protocol for TH antibody 66334-1-IgDownload protocol
IF protocol for TH antibody 66334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Neurol

PMID: 30949126

Authors - Xingfang Guo
ratIHC

CNS Neurosci Ther

Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.

Authors - Yuan Qian
humanWB

Exp Ther Med

Wnt1 silencing enhances neurotoxicity induced by paraquat and maneb in SH-SY5Y cells.

Authors - Cui Huang
mouseWB,IHC

Neurosci Lett

Inhibition of HDAC6 increases acetylation of peroxiredoxin1/2 and ameliorates 6-OHDA induced dopaminergic injury.

Authors - Wencheng Jian
humanIF

Biochem Biophys Res Commun

Mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of a child with Rett syndrome.

Authors - Saki Hirofuji
humanWB

Eur J Pharmacol

Asparagine endopeptidase inhibitor protects against fenpropathrin-induced neurodegeneration via suppressing α-synuclein aggregation and neuroinflammation.

Authors - Ting Yu