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TIMM44 Monoclonal antibody
TIMM44 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2c
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4G7D4
Cat no : 66149-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rabbit heart tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66149-1-Ig targets TIMM44 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Reactivity | human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG2c |
Class | Monoclonal |
Type | Antibody |
Immunogen | TIMM44 fusion protein Ag21389 |
Full Name | translocase of inner mitochondrial membrane 44 homolog (yeast) |
Calculated Molecular Weight | 452 aa, 51 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC033628 |
Gene Symbol | TIMM44 |
Gene ID (NCBI) | 10469 |
RRID | AB_2881545 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for TIMM44 antibody 66149-1-Ig | Download protocol |
IHC protocol for TIMM44 antibody 66149-1-Ig | Download protocol |
IF protocol for TIMM44 antibody 66149-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Lana (Verified Customer) (04-20-2019) | SDS-PAGE:15 ug/ul RIPA lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 800CW Goat anti-Mouse1 h incubation at room temperatureLines on WB:1. BioRad Precision Plus Protein standards2. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line
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