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TMED9 Polyclonal antibody

TMED9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21620-1-AP

Synonyms

Glycoprotein 25L2, GMP25, GP25L2, HSGP25L2G, TMED9



Tested Applications

Positive WB detected inL02 cells, rat liver tissue, mouse pancreas tissue, mouse lung tissue, mouse liver tissue, mouse brain tissue, pig liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21620-1-AP is TMED9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMED9 fusion protein Ag13813
Full Name transmembrane emp24 protein transport domain containing 9
Calculated molecular weight 235 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001123
Gene symbol TMED9
Gene ID (NCBI) 54732
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

Protocols

Product Specific Protocols
WB protocol for TMED9 antibody 21620-1-APDownload protocol
IHC protocol for TMED9 antibody 21620-1-APDownload protocol
IF protocol for TMED9 antibody 21620-1-APDownload protocol
IP protocol for TMED9 antibody 21620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Authors - Moran Dvela-Levitt
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated