TMEM192 Polyclonal antibody

TMEM192 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28263-1-AP

Synonyms

FLJ38482, TMEM192, transmembrane protein 192



Tested Applications

Positive WB detected inHeLa cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28263-1-AP targets TMEM192 in WB, IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM192 fusion protein Ag27576
Full Name transmembrane protein 192
Observed molecular weight 30 kDa
GenBank accession numberBC036301
Gene symbol TMEM192
Gene ID (NCBI) 201931
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or was detected at low levels in normal cell lines.(PMID: 22736246) TMEM192 can be detect as 30 kDa.

Protocols

Product Specific Protocols
WB protocol for TMEM192 antibody 28263-1-APDownload protocol
IHC protocol for TMEM192 antibody 28263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (09-27-2020)

This antibody is very good, and I recommend this to others who are doing lyso-IP using a tagged TMEM192.

  • Applications: Western Blot,Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
TMEM192 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HEK293T (Cat no:28263-1-AP)