TMEM70 Monoclonal antibody

TMEM70 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2C11C3

Cat no : 60195-1-Ig

Synonyms

TMEM70, transmembrane protein 70



Tested Applications

Positive WB detected inA2780 cells
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 60195-1-Ig is TMEM70 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMEM70 fusion protein Ag16947
Full Name transmembrane protein 70
Calculated molecular weight 260 aa, 29 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002748
Gene symbol TMEM70
Gene ID (NCBI) 54968
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication (PMID:21147908) identified TMEM70 gene defect as a pan-ethnic disorder and further redefined it as the most common cause of nuclear-origin ATP synthase deficiency.

Protocols

Product Specific Protocols
WB protocol for TMEM70 antibody 60195-1-IgDownload protocol
IHC protocol for TMEM70 antibody 60195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mitochondrion

Mitochondrial membrane assembly of TMEM70 protein.

Authors - Hana Kratochvílová