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TNF alpha Antibody 62 Publications

Mouse Monoclonal| Catalog number: 60291-1-Ig |CloneNo.: 7B8A11

Featured Product KD/KO validated

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Con: 222 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

TNF alpha Antibody WB LPS treated RAW 264.7 cells 60291-1-Ig

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TNF alpha Antibody WB HeLa cells 60291-1-Ig

WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells

TNF alpha Antibody IHC human breast cancer tissue 60291-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TNF alpha Antibody IHC human breast cancer tissue 60291-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TNF alpha Antibody IF human breast cancer tissue 60291-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

TNF alpha Antibody IF human breast cancer tissue 60291-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, chicken
Positive WB detected in:
LPS treated RAW 264.7 cells, HeLa cells, HL-60 cells, PC-12 cells
Positive IHC detected in:
human breast cancer tissue, human colon cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human breast cancer tissue
Recommended dilution:
WB : 1:2000-1:5000
IHC : 1:200-1:1000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TNF alpha fusion protein Ag11413
Full name:
tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight:
233aa,26 kDa
Observed molecular weight:
17-22 kDa
GenBank accession number:
BC028148
Gene ID (NCBI):
7124
Gene symbol
TNF
Synonyms
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, TNFα, Tumor necrosis factor

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.


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