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TNF alpha Antibody
TNF alpha Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
7B8A11
Cat no : 60291-1-Ig
Synonyms
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, TNFα, Tumor necrosis factor
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-12 cells, HeLa cells, U-937 cells, LPS treated RAW 264.7 cells, 4T1 cells, RAW 264.7 cells, HSC-T6 cells, HL-60 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 71 publications below |
| IHC | See 17 publications below |
| IF | See 8 publications below |
| ELISA | See 1 publications below |
| FC | See 1 publications below |
Product Information
60291-1-Ig targets TNF alpha in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | chicken, human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TNF alpha fusion protein Ag11413 |
| Full Name | tumor necrosis factor (TNF superfamily, member 2) |
| Calculated molecular weight | 233aa,26 kDa |
| Observed molecular weight | 17-22 kDa |
| GenBank accession number | BC028148 |
| Gene symbol | TNF |
| Gene ID (NCBI) | 7124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNF alpha antibody 60291-1-Ig | Download protocol |
| IHC protocol for TNF alpha antibody 60291-1-Ig | Download protocol |
| IF protocol for TNF alpha antibody 60291-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Drug Des Devel Ther Beraprost sodium preconditioning prevents inflammation, apoptosis, and autophagy during hepatic ischemia-reperfusion injury in mice via the P38 and JNK pathways. | ||
Neurochem Res Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission. | ||
Transpl Immunol Protective effect of rosiglitazone on chronic renal allograft dysfunction in rats. | ||
Biomed Pharmacother Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. | ||
J Exp Med Thyrotropin aggravates atherosclerosis by promoting macrophage inflammation in plaques. | ||
Mol Med Rep miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Feng-Qian (Verified Customer) (12-07-2018) | Signal was very weak.
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