Name: TNF alpha Mouse Monoclonal antibody
Brand: Proteintech
SKU: 60291-1-Ig

Tested Applications

Applications:

WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee

TNF alpha Antibody WB LPS treated RAW 264.7 cells 60291-1-Ig

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TNF alpha Antibody WB HeLa cells 60291-1-Ig

WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells

TNF alpha Antibody IHC human breast cancer tissue 60291-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TNF alpha Antibody IF human breast cancer tissue 60291-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:

human, mouse, rat, chicken

Positive WB detected in:

LPS treated RAW 264.7 cells, HeLa cells, PC-12 cells, U-937 cells, HL-60 cells, RAW 264.7 cells, HSC-T6 cells, 4T1 cells

Positive IHC detected in:

human breast cancer tissue, human liver cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

human breast cancer tissue

Recommended dilution:

WB : 1:2000-1:5000

IHC : 1:200-1:1000

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Product Information

Source:

Mouse

Purification method:

Protein G purification

Isotype:

IgG2b

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

TNF alpha fusion protein Ag11413

Full name:

tumor necrosis factor (TNF superfamily, member 2)

Calculated molecular weight:

233aa,26 kDa

Observed molecular weight:

17-22 kDa

GenBank accession number:

BC028148

Gene ID (NCBI):

7124

Gene symbol

TNF

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, TNFα, Tumor necrosis factor

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Showing 1- [6] of [73] Publications

Author:		Yang Wu
Pubmed ID:		24813629
Journal:		FEBS Lett
Title:		MiR-152 reduces human umbilical vein endothelial cell proliferation and migration by targeting ADAM17.
Application:		WB

Species:		human

Author:		Xiaomin Xia
Pubmed ID:		25046589
Journal:		Int Immunopharmacol
Title:		Role of α-lipoic acid in LPS/d-GalN induced fulminant hepatic failure in mice: Studies on oxidative stress, inflammation and apoptosis.
Application:		WB

Species:		mouse

Author:		Demei Xu
Pubmed ID:		25123790
Journal:		Toxicol Appl Pharmacol
Title:		Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: The prevention of curcumin.
Application:		WB

Species:		mouse

Author:		Danfeng Wang
Pubmed ID:		25928088
Journal:		Chronobiol Int
Title:		Maternal obesity disrupts circadian rhythms of clock and metabolic genes in the offspring heart and liver.
Application:		WB

Species:		mouse

Author:		Yue Zhai
Pubmed ID:		26296700
Journal:		J Mol Med (Berl)
Title:		CD147 promotes IKK/IκB/NF-κB pathway to resist TNF-induced apoptosis in rheumatoid arthritis synovial fibroblasts.
Application:		IHC

Species:		human

Author:		Chuan Wang
Pubmed ID:		26311557
Journal:		Placenta
Title:		The effect of maternal obesity on the expression and functionality of placental P-glycoprotein: Implications in the individualized transplacental digoxin treatment for fetal heart failure.
Application:		WB

Species:		human

WB Figures

TNF alpha Antibody WB 4T1 cells 60291-1-Ig

4T1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells

TNF alpha Antibody WB HL-60 cells 60291-1-Ig

HL-60 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TNF alpha Antibody WB HSC-T6 cells 60291-1-Ig

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TNF alpha Antibody WB PC-12 cells 60291-1-Ig

PC-12 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

PC-12 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

TNF alpha Antibody WB RAW 264.7 cells 60291-1-Ig

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

TNF alpha Antibody WB U-937 cells 60291-1-Ig

U-937 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

TNF alpha Antibody IHC human colon cancer tissue 60291-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:50 (under 40x lens)

TNF alpha Antibody IHC human liver cancer tissue 60291-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60291-1-Ig( TNF alpha antibody at dilution of 1:50 (under 40x lens)

IF Figures

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

TNF alpha Antibody 73 Publications

Mouse Monoclonal| Catalog number: 60291-1-Ig |CloneNo.: 7B8A11

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [73] Publications

WB Figures

IHC Figures

IF Figures

Product-specific protocols

Standard Protocols

TNF alpha Antibody 73 Publications

Mouse Monoclonal| Catalog number: 60291-1-Ig |CloneNo.: 7B8A11

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Tested Applications

Product Information

Immunogen Information

Background