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  • KD/KO Validated

TNF Alpha Monoclonal antibody

TNF Alpha Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

7B8A11

Cat no : 60291-1-Ig

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, TNFα, Tumor necrosis factor



Tested Applications

Positive WB detected inPC-12 cells, HeLa cells, U-937 cells, LPS treated RAW 264.7 cells, 4T1 cells, RAW 264.7 cells, HSC-T6 cells, HL-60 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60291-1-Ig targets TNF Alpha in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity chicken, human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TNF Alpha fusion protein Ag11413
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC028148
Gene symbol TNF
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Protocols

Product Specific Protocols
WB protocol for TNF Alpha antibody 60291-1-IgDownload protocol
IHC protocol for TNF Alpha antibody 60291-1-IgDownload protocol
IF protocol for TNF Alpha antibody 60291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Drug Des Devel Ther

Beraprost sodium preconditioning prevents inflammation, apoptosis, and autophagy during hepatic ischemia-reperfusion injury in mice via the P38 and JNK pathways.

Authors - Jingfan Deng
mouseWB

Neurochem Res

Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission.

Authors - Siyu Chen
ratWB

Transpl Immunol

PMID: 30682409

Authors - Jin Deng
mouseWB

Biomed Pharmacother

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2.

Authors - Fanchao Feng
mouseIHC

J Exp Med

Thyrotropin aggravates atherosclerosis by promoting macrophage inflammation in plaques.

Authors - Chongbo Yang
mouseWB

Mol Med Rep

miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN.

Authors - Si Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Feng-Qian (Verified Customer) (12-07-2018)

Signal was very weak.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1/250
  • Cell Tissue Type: HaCaT