Tested Applications
| Positive WB detected in | HeLa, K-562 cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11278-1-AP targets TOM22 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1797 Product name: Recombinant human TOMM22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC009363 Sequence: MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPSLPGKI Predict reactive species |
| Full Name | translocase of outer mitochondrial membrane 22 homolog (yeast) |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15-22 kDa |
| GenBank Accession Number | BC009363 |
| Gene Symbol | TOMM22/Tom22 |
| Gene ID (NCBI) | 56993 |
| RRID | AB_2207542 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NS69 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises the 22 kDa protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOM22 antibody 11278-1-AP | Download protocol |
| IHC protocol for TOM22 antibody 11278-1-AP | Download protocol |
| WB protocol for TOM22 antibody 11278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TOMM20 antibody (Cat# 11278-1-AP) has 14 citations across journals including Cell Research, Cell Discovery, Advanced Science, Cancer Letters, PNAS, EMBO Reports, mBio, and Molecular Oncology. Research fields span mitochondrial biology, ferroptosis, mitophagy, pyroptosis, cancer stemness, neurodegeneration (Alzheimer's), cardiomyopathy, and SARS-CoV-2 infection. Applications are predominantly WB, with IF, IP, and CoIP also represented.
| Species | Application | Title |
|---|---|---|
Cell Discov Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis. | ||
Adv Sci (Weinh) SENP6 Maintains Mitochondrial Homeostasis by Regulating Mitochondrial Protein Import Through deSUMOylation of TOM40 | ||
Cancer Lett The BTN3A3-TOMM22 axis preserves mitochondrial homeostasis to facilitate HCC stemness and drug resistance.
| ||
Proc Natl Acad Sci U S A Mitochondrial ROS triggers mitophagy through activating the DNA damage response signaling pathway. | ||
JACC Basic Transl Sci Bag3 Regulates Mitochondrial Function and the Inflammasome Through Canonical and Noncanonical Pathways in the Heart |
