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TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11741-1-AP

Synonyms

C20orf1, C20orf2, DIL 2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, p100, Protein fls353, REPP86, Targeting protein for Xklp2, TPX2



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11741-1-AP targets TPX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPX2 fusion protein Ag2334
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC020207
Gene symbol TPX2
Gene ID (NCBI) 22974
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TPX2 antibody 11741-1-APDownload protocol
IHC protocol for TPX2 antibody 11741-1-APDownload protocol
IF protocol for TPX2 antibody 11741-1-APDownload protocol
IP protocol for TPX2 antibody 11741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
humanWB,IF

BMC Biol

CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment.

Authors - Haining Zhou
mouseWB

J Cell Sci

Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility.

Authors - Meng-Wen Hu
humanIF

J Cell Sci

Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation.

Authors - Haijing Guo
humanIHC

Front Physiol

Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling.

Authors - Baozhen Sun
humanWB

Sci Rep

Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.

Authors - Helin Wang