transgelin/SM22-specific Polyclonal antibody

transgelin/SM22-specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15502-1-AP

Synonyms

SM22, SM22 alpha, SM22a, SM22alpha, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1



Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:100-1:4000
Sample-dependent, check data in validation data gallery

Product Information

15502-1-AP targets transgelin/SM22-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberNM_003186
Gene symbol TAGLN
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.

Protocols

Product Specific Protocols
WB protocol for transgelin/SM22-specific antibody 15502-1-APDownload protocol
IHC protocol for transgelin/SM22-specific antibody 15502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols