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  • KD/KO Validated

TRBP Antibody

TRBP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 15753-1-AP

Synonyms

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, mouse spleen tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15753-1-AP targets TRBP in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRBP fusion protein Ag8255
Full Name TAR (HIV-1) RNA binding protein 2
Calculated molecular weight 39 kDa
Observed molecular weight 43-46 kDa
GenBank accession number BC005860
Gene symbol TARBP2
Gene ID (NCBI) 6895
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43–46 kDa, are expressed in human cells. (PMID: 16142218)

Protocols

Product Specific Protocols
WB protocol for TRBP antibody 15753-1-APDownload protocol
IHC protocol for TRBP antibody 15753-1-APDownload protocol
IF protocol for TRBP antibody 15753-1-APDownload protocol
IP protocol for TRBP antibody 15753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nature

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Authors - Mathieu Ringeard
humanWB, IP

Mol Biol Cell

A Biosensor for MAPK-Dependent Lin28 Signaling.

Authors - Laurel M Oldach
humanWB

Biochem Biophys Res Commun

The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.

Authors - Akihiko Komuro
  • KD Validated
humanWB,IF

Nucleic Acids Res

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Authors - Matthew J Warner
  • KD Validated
humanWB

Nat Commun

SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.

Authors - Cheng Chen
  • KD Validated
mouseWB

Mol Cell

A Rapid Induction Mechanism for Lin28a in Trophic Responses.

Authors - Alexandra M Amen