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TRIB2 Polyclonal antibody

TRIB2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15359-1-AP

Synonyms

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse thymus tissue, K-562 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15359-1-AP targets TRIB2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIB2 fusion protein Ag7605
Full Name tribbles homolog 2 (Drosophila)
Calculated molecular weight 39 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002637
Gene symbol TRIB2
Gene ID (NCBI) 28951
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

Protocols

Product Specific Protocols
WB protocol for TRIB2 antibody 15359-1-APDownload protocol
IHC protocol for TRIB2 antibody 15359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Exp Mol Med

Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.

Authors - Eun Kyoung Do
  • KD Validated