Tested Applications
| Positive WB detected in | A549 cells, U-937 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67171-1-Ig targets TRIB2 in WB, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7501 Product name: Recombinant human TRIB2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-343 aa of BC002637 Sequence: MNIHRSTPITIARYGRSRNKTQDFEELSSIRSAEPSQSFSPNLGSPSPPETPNLSHCVSCIGKYLLLEPLEGDHVFRAVHLHSGEELVCKVFDISCYQESLAPCFCLSAHSNINQITEIILGETKAYVFFERSYGDMHSFVRTCKKLREEEAARLFYQIASAVAHCHDGGLVLRDLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLSDKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTMLVGRYPFHDIEPSSLFSKIRRGQFNIPETLSPKAKCLIRSILRREPSERLTSQEILDHPWFSTDFSVSNSAYGAKEVSDQLVPDVNMEENLDPFFN Predict reactive species |
| Full Name | tribbles homolog 2 (Drosophila) |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC002637 |
| Gene Symbol | TRIB2 |
| Gene ID (NCBI) | 28951 |
| RRID | AB_2882467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q92519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIB2 antibody 67171-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIB2 Monoclonal antibody (1A10A6), catalog number 67171-1-Ig, has 2 total citations. These appear in Free Radical Biology and Medicine (2026) and Structure (2022). The research fields covered include pulmonary hypertension—specifically TRIB2-mediated pulmonary artery smooth muscle cell proliferation via SERCA2 ubiquitination—and structural biology of the TRIB2 pseudokinase, where nanobodies were used to identify its activated conformational state. Both studies utilized the antibody for co-immunoprecipitation in human samples.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med TRIB2 promotes pulmonary artery smooth muscle cell proliferation through SERCA2 ubiquitination in pulmonary hypertension. | ||
Reviews
What customers say
The antibody received a 4-star rating from a user who tested it for flow cytometry at a 1:50 dilution. The reviewer reported that the antibody works well for FACS applications, confirming its reliability in this use case. Overall, the single review reflects a positive experience with the product's performance in flow cytometry.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ridhima (Verified Customer) (07-12-2021) | The antibody works well for FACS .
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