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  • KD/KO Validated

TRIM14 Polyclonal antibody

TRIM14 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15742-1-AP

Synonyms

KIAA0129, TRIM14, tripartite motif containing 14



Tested Applications

Positive WB detected inL02 cells, HL-60 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15742-1-AP targets TRIM14 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM14 fusion protein Ag8158
Full Name tripartite motif-containing 14
Calculated molecular weight 442 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC006333
Gene symbol TRIM14
Gene ID (NCBI) 9830
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

Protocols

Product Specific Protocols
WB protocol for TRIM14 antibody 15742-1-APDownload protocol
IHC protocol for TRIM14 antibody 15742-1-APDownload protocol
IF protocol for TRIM14 antibody 15742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

ESMO Open

Immunohistochemical validation study of 15-gene biomarker panel predictive of benefit from adjuvant chemotherapy in resected non-small-cell lung cancer: analysis of JBR.10.

Authors - Stacy Grieve
humanWB

Sci Rep

TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.

Authors - Josephine Hai
  • KD Validated
humanWB

Sci Rep

TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway.

Authors - Guoxing Xu
  • KD Validated
humanWB

Sci Rep

Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation.

Authors - Mei-Xiu Jiang