Tested Applications
| Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82345-4-RR targets TRIM63 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tripartite motif-containing 63 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | NM_032588 |
| Gene Symbol | TRIM63 |
| Gene ID (NCBI) | 84676 |
| RRID | AB_3743403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q969Q1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM63 antibody 82345-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM63 Recombinant monoclonal antibody (242055A4), catalog number 82345-4-RR, has 1 total citation. It appears in Free Radical Biology and Medicine (IF: 8), published in 2026. The cited study investigates how USP47 stabilizes HDAC2 to ameliorate cigarette smoke-induced skeletal muscle atrophy by suppressing CYP1A1/ROS-mediated autophagy. The research fields encompass skeletal muscle atrophy, oxidative stress, autophagy regulation, and E3 ubiquitin ligase function. The antibody was applied in Western blot using mouse samples.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med USP47 stabilizes HDAC2 to ameliorate cigarette smoke-induced skeletal muscle atrophy by suppressing CYP1A1/ROS-mediated autophagy. |
