Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, A549 cells, MCF-7 cells, U2OS cells, NIH/3T3 cells, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86095-1-RR targets TRMU in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag21930 Product name: Recombinant human TRMU protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-267 aa of BC027991 Sequence: MKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL Predict reactive species |
| Full Name | tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase |
| Calculated Molecular Weight | 421 aa, 48 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC027991 |
| Gene Symbol | TRNT1 |
| Gene ID (NCBI) | 55687 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75648 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRMU (tRNA m5C methyltransferase) is a mitochondrial protein involved in the modification of mitochondrial tRNA. Specifically, it catalyzes the formation of 5-methylcytidine at position 34 (m5C34) in the anticodon stem-loop of mitochondrial tRNAs, which is crucial for maintaining the stability and function of these tRNAs. TRMU is a nuclear-encoded protein located in the mitochondrial matrix. It plays a vital role in mitochondrial translation and oxidative phosphorylation.TRMU is associated with several genetic disorders. Mutations in the TRMU gene can cause mitochondrial diseases, such as infantile hepatic failure syndrome. (PMID:23625533)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRMU antibody 86095-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
