|Positive WB detected in||HeLa cells, mouse brain tissue, mouse cerebellum tissue|
|Positive IHC detected in||human testis tissue, human brain tissue, human kidney tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14555-1-AP targets Alpha Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Tubulin fusion protein Ag6038|
|Full Name||tubulin, alpha 1a|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC050637|
|Gene ID (NCBI)||7846|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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