Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14555-1-AP

Synonyms

alpha tubulin, TUBA1A, TUBA3



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14555-1-AP targets Alpha Tubulin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag6038
Full Name tubulin, alpha 1a
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC050637
Gene symbol TUBA1A
Gene ID (NCBI) 7846
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nanomedicine

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.

Authors - Adel Al Fatease
humanWB

Cell Rep

SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.

Authors - Shufen Li
humanWB,IF

Exp Cell Res

Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.

Authors - Shen Enzhi E
mouseWB

Biochim Biophys Acta Mol Basis Dis

Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.

Authors - Yan Wang
humanWB

J Cell Sci

ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.

Authors - Mohamed Bourmoum