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Thymidylate synthase Polyclonal antibody

Thymidylate synthase Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 15047-1-AP

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS



Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HEK-293 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15047-1-AP targets Thymidylate synthase in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Thymidylate synthase fusion protein Ag7027
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC002567
Gene symbol TYMS
Gene ID (NCBI) 7298
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Protocols

Product Specific Protocols
WB protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
IHC protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
IF protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
IP protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
FC protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

N/A

Screening for Target Engagement using the Cellular Thermal Shift Assay - CETSA

Authors - G. Sitta Sittampalam
WB

Vet Comp Oncol

Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.

Authors - Shih-Chieh Chang
humanWB

Mol Med Rep

PMID: 30535504

Authors - Changwen Jing
humanWB

Tumour Biol

Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.

Authors - Bo Liao
humanIHC

Ann Transl Med

Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.

Authors - Wenhui Xie
human

PLoS One

An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.

Authors - Yan Ting Lim