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- KD/KO Validated
Thymidylate synthase Polyclonal antibody
Thymidylate synthase Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 15047-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293T cells, HEK-293 cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human endometrial cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15047-1-AP targets Thymidylate synthase in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Thymidylate synthase fusion protein Ag7027|
|Full Name||thymidylate synthetase|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||32-36 kDa|
|GenBank accession number||BC002567|
|Gene ID (NCBI)||7298|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.
|Product Specific Protocols|
|WB protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|IHC protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|IF protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|IP protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|FC protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Screening for Target Engagement using the Cellular Thermal Shift Assay - CETSA
Vet Comp Oncol
Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.
Mol Med Rep
Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.
Ann Transl Med
Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.
An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.