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TYMS Monoclonal antibody

TYMS Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1F5B7

Cat no : 66725-1-Ig

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66725-1-Ig targets TYMS in WB, IHC, FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TYMS fusion protein Ag7027
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002567
Gene symbol TYMS
Gene ID (NCBI) 7298
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Protocols

Product Specific Protocols
WB protocol for TYMS antibody 66725-1-IgDownload protocol
IHC protocol for TYMS antibody 66725-1-IgDownload protocol
FC protocol for TYMS antibody 66725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (03-26-2021)

Not working

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver, mouse brain / HepG2
TYMS Antibody Western Blot validation (1:1000 dilution) in Mouse liver, mouse brain / HepG2 (Cat no:66725-1-Ig)