HRP-conjugated TYMS Monoclonal antibody

TYMS Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

HRP Fluorescent Dye

CloneNo.

1F5B7

Cat no : HRP-66725

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS



Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

HRP-66725 targets TYMS in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TYMS fusion protein Ag7027
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002567
Gene symbol TYMS
Gene ID (NCBI) 7298
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Protocols

Product Specific Protocols
WB protocol for HRP TYMS antibody HRP-66725Download protocol
Standard Protocols
Click here to view our Standard Protocols