Tubulin Beta Class V Monoclonal antibody

Tubulin Beta Class V Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, dog

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D7B9

Cat no : 66362-1-Ig

Synonyms

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66362-1-Ig targets Tubulin Beta Class V in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, dog samples.

Tested Reactivity human, mouse, dog
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 6
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_032525
Gene symbol TUBB6
Gene ID (NCBI) 84617
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.

Protocols

Product Specific Protocols
WB protocol for Tubulin Beta Class V antibody 66362-1-IgDownload protocol
IHC protocol for Tubulin Beta Class V antibody 66362-1-IgDownload protocol
IF protocol for Tubulin Beta Class V antibody 66362-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Neurobiol

Alpha- and beta-tubulin isotypes are differentially expressed during brain development.

Authors - Torben J Hausrat