UBC9 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 60201-1-Ig |CloneNo.: 4A10B11

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Con: 180 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
RAW 264.7 cells, HEK-293 cells, rat kidney tissue

Positive IHC detected in:
human lymphoma tissue

Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:200-1:600

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2a

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)

Calculated molecular weight:
18 kDa

Observed molecular weight:
18 kDa, 54 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
UBE2I

Synonyms
C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I
Background

UBE2I is also named as UBC9, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).


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