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UBE2E3 Polyclonal antibody

UBE2E3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15488-1-AP

Synonyms

UBCE4, UBCH9, UbcM2, UBE2E3, Ubiquitin carrier protein E3, Ubiquitin protein ligase E3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using UBE2E3 Polyclonal antibody (15488-1-AP)

    WB analysis of Jurkat using 15488-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using UBE2E3 Polyclonal antibody (15488-1-AP)

    WB analysis of Y79 using 15488-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    WB analysis of mouse liver using 15488-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    WB analysis of mouse liver using 15488-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    WB analysis of mouse liver using 15488-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    WB analysis of mouse kidney using 15488-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    IHC staining of human heart using 15488-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    IHC staining of human heart using 15488-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    IHC staining of human skeletal muscle using 15488-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using UBE2E3 Polyclonal antibody (15488-1-AP)

    IHC staining of human skeletal muscle using 15488-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, mouse liver tissue, Y79 cells
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15488-1-AP targets UBE2E3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2E3 fusion protein Ag7838
Full Name ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC003554
Gene Symbol UBE2E3
Gene ID (NCBI) 10477
RRIDAB_10896456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBE2E3 antibody 15488-1-APDownload protocol
IHC protocol for UBE2E3 antibody 15488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuropathology

Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.

Authors - Kazuhiko Watabe
View Article
mouseWB

Int J Mol Sci

miR-143-3p Promotes Ovarian Granulosa Cell Senescence and Inhibits Estradiol Synthesis by Targeting UBE2E3 and LHCGR

Authors - Jingxian Deng
View Article