Tested Applications
| Positive WB detected in | HEK-293 cells, rat brain tissue, HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23449-1-AP targets Ubiquilin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20149 Product name: Recombinant human Ubiquilin 2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-624 aa of BC069237 Sequence: MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS Predict reactive species |
| Full Name | ubiquilin 2 |
| Calculated Molecular Weight | 624 aa, 66 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC069237 |
| Gene Symbol | UBQLN2 |
| Gene ID (NCBI) | 29978 |
| RRID | AB_2879282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHD9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquilin-2 (UBQLN2) is a multifunctional, ubiquitin-binding shuttle protein that plays a central role in maintaining cellular protein homeostasis. It is a member of the ubiquilin family of proteins found in all eukaryotes, which function to regulate the degradation of ubiquitinated proteins. Of the five different UBQLN genes in humans, UBQLN2 is the most highly expressed in the nervous system and muscle tissue and has been linked to multiple neurodegenerative diseases. (PMID: 40663766, PMID: 40663766)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ubiquilin 2 antibody 23449-1-AP | Download protocol |
| IHC protocol for Ubiquilin 2 antibody 23449-1-AP | Download protocol |
| WB protocol for Ubiquilin 2 antibody 23449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ubiquilin 2 Polyclonal antibody (Cat# 23449-1-AP) has 13 citations published in journals including Nature Protocols, Brain, EMBO Journal, Journal of Ethnopharmacology, Neurobiology of Disease, Molecular Neurobiology, Brain Pathology, Journal of Neurochemistry, Biophysical Journal, Human Molecular Genetics, F1000Research, and bioRxiv. Research fields span neurodegenerative diseases (ALS/FTD, vascular dementia, Parkinsonism), muscle pathology, protein phase separation, extracellular vesicle biology, and cardiomyocyte apoptosis.
| Species | Application | Title |
|---|---|---|
Brain Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies | ||
J Ethnopharmacol Huanglian-ejiao decoction ameliorates doxorubicin-induced cardiomyocyte apoptosis and autophagic flux dysregulation by up-regulating ubiquilin1. | ||
Neurobiol Dis Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers. | ||
Mol Neurobiol Synaptic Vesicle-Related Proteins and Ubiquilin 2 in Cortical Synaptosomes Mediate Cognitive Impairment in Vascular Dementia Rats |
Reviews
What customers say
One user reviewed this product with a 5-star rating, reporting positive results in Western Blot on 293T cells at a 1:1000 dilution. The reviewer described the antibody quality as good. Overall, the feedback is favorable, with the single review highlighting reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xingyuan (Verified Customer) (06-22-2023) | Antibody quality was good.
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