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  • KD/KO Validated

VAPB Polyclonal antibody

VAPB Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14477-1-AP

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB



Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, HeLa cells, mouse skeletal muscle tissue, HepG2 cells, Jurkat cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14477-1-AP targets VAPB in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAPB fusion protein Ag5857
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001712
Gene symbol VAPB
Gene ID (NCBI) 9217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Protocols

Product Specific Protocols
WB protocol for VAPB antibody 14477-1-APDownload protocol
IHC protocol for VAPB antibody 14477-1-APDownload protocol
IF protocol for VAPB antibody 14477-1-APDownload protocol
IP protocol for VAPB antibody 14477-1-APDownload protocol
FC protocol for VAPB antibody 14477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Neurosci Bull

Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.

Authors - Dan Wu
  • KD Validated
mouseWB,IP

Sci Rep

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Authors - Tomoyuki Yamanaka
mouseWB

J Biol Chem

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

Authors - Pamela A Young
mouseWB,IHC

Mol Neurodegener

Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.

Authors - Qiu Linghua L
humanWB

Eur J Neurol

Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.

Authors - I Deidda

J Virol

Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.

Authors - Nicholas L Cianciola

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (09-22-2020)

The antibody have predicted band on western blot.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse heart