Validation Data Gallery
|Positive WB detected in||mouse brain tissue, mouse liver tissue, HeLa cells, mouse skeletal muscle tissue, HepG2 cells, Jurkat cells, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14477-1-AP targets VAPB in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAPB fusion protein Ag5857|
|Full Name||VAMP (vesicle-associated membrane protein)-associated protein B and C|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC001712|
|Gene ID (NCBI)||9217|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
|Product Specific Protocols|
|WB protocol for VAPB antibody 14477-1-AP||Download protocol|
|IHC protocol for VAPB antibody 14477-1-AP||Download protocol|
|IF protocol for VAPB antibody 14477-1-AP||Download protocol|
|IP protocol for VAPB antibody 14477-1-AP||Download protocol|
|FC protocol for VAPB antibody 14477-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
J Biol Chem
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Eur J Neurol
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (09-22-2020)
The antibody have predicted band on western blot.