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VEGF Antibody 80 Publications

Rabbit Polyclonal| Catalog number: 19003-1-AP

Featured Product KD/KO validated

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Con: 55 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IHC, IP, WB
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Images See all

VEGF Antibody WB PC-3 cells 19003-1-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP(VEGF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

VEGF Antibody IHC human lung cancer tissue 19003-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 19003-1-AP( VEGF antibody at dilution of 1:200 (under 10x lens).

VEGF Antibody IHC human lung cancer tissue 19003-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 19003-1-AP( VEGF antibody at dilution of 1:200 (under 40x lens).

VEGF Antibody IF HeLa cells 19003-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 19003-1-AP(VEGF antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

VEGF Antibody IP MCF-7 cells 19003-1-AP

IP Result of anti-VEGFA (IP:19003-1-AP, 3ug; Detection:19003-1-AP 1:500) with MCF-7 cells lysate 2500ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
PC-3 cells, A431 cells, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human lung cancer tissue,human placenta tissue, human liver cancer tissue, human kidney tissue, human colon cancer tissue, human oesophagus tissue
Positive IF detected in:
HeLa cells, HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
VEGF fusion protein Ag13500
Full name:
vascular endothelial growth factor A
Calculated molecular weight:
412aa,46 kDa
Observed molecular weight:
23-27 kDa,45 kDa
GenBank accession number:
BC065522
Gene ID (NCBI):
7422
Gene symbol
VEGFA
Synonyms
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Background

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010 ). This antibody can recognize all the isoforms of VEFGA.


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