Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 19003-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 19003-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 19003-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 19003-1-AP
bEnd.3 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 19003-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 19003-1-AP
IP result of anti-VEGFA (IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.
IHC staining of human lung cancer using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19003-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 19003-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 19003-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of NIH/3T3 using 19003-1-AP
1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

VEGFA Polyclonal antibody

The VEGFA polyclonal antibody (19003-1-AP) is one of the most highly cited VEGFA antibodies in the market. It is suitable for WB, IHC, IF/ICC, FC (Intra), IP, ELISA and has been referenced in over 897 publications.

Cat No. 19003-1-AP

Publications(897)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

VEGF, L-VEGF, N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (897)
Protocols
Reviews (3)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, bEnd.3 cells, C6 cells, Jurkat cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 17 publications below
WB	See 654 publications below
IHC	See 267 publications below
IF	See 112 publications below
IP	See 1 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

19003-1-AP targets VEGFA in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, monkey, bovine, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VEGFA fusion protein Ag13500 Predict reactive species
Full Name	vascular endothelial growth factor A
Calculated Molecular Weight	412 aa, 46 kDa
Observed Molecular Weight	36-40 kDa
GenBank Accession Number	BC065522
Gene Symbol	VEGFA
Gene ID (NCBI)	7422
RRID	AB_2212657
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15692
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Protocols

Product Specific Protocols
WB protocol for VEGFA antibody 19003-1-AP	Download protocol
IHC protocol for VEGFA antibody 19003-1-AP	Download protocol
IF protocol for VEGFA antibody 19003-1-AP	Download protocol
IP protocol for VEGFA antibody 19003-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cancer Cell Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis Authors - Jiang Chang View Article
mouse	WB,IHC	Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades. Authors - Xuefei Lu KD Validated View Article
human	WB	Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds Authors - Shunlai Shang View Article
mouse,human	WB	ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. Authors - Meng Zhao View Article
human	WB	Adv Sci (Weinh) Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer Authors - Jindong Xie View Article
mouse	IHC	Biomaterials A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration Authors - Congcong Yu View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Angie (Verified Customer) (07-24-2024)	WB result of VEGFA antibody (used at 1:5000) incubated at room temperature for 1.5 hours. Two stronger bands between 35-50 kDa were observed. Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: A549 VEGFA Antibody Western Blot validation (1:5000 dilution) in A549 (Cat no:19003-1-AP)
FH Alexandru (Verified Customer) (11-21-2023)	Great antibody with clear and specific signal, would buy again! Applications: Western Blot Cell Tissue Type: Mouse Ventricle
FH Fiona (Verified Customer) (07-16-2019)	Good quality antibody and good value for money. Would purchase again. Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: ARPE-19 VEGFA Antibody Western Blot, validation (1:500 dilution) in ARPE-19 (Cat no:19003-1-AP)