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Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 10366-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, Raji cells, Jurkat cells, mouse heart tissue, NIH/3T3 cells|
|Positive IHC detected in||human liver cancer tissue, human colon tissue, human tonsillitis tissue, human ovary tumor tissue, human liver tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse brain tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1500-1:6000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10366-1-AP targets Vimentin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sika deer|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Vimentin fusion protein Ag0489|
|Calculated molecular weight||466aa,54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC000163|
|Gene ID (NCBI)||7431|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
|Product Specific Protocols|
|WB protocol for Vimentin antibody 10366-1-AP||Download protocol|
|IHC protocol for Vimentin antibody 10366-1-AP||Download protocol|
|IF protocol for Vimentin antibody 10366-1-AP||Download protocol|
|FC protocol for Vimentin antibody 10366-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
wei (Verified Customer) (04-27-2020)
I tried in C2C12 cells
Patricia (Verified Customer) (02-25-2020)
Kishor (Verified Customer) (01-30-2019)
Good results in western blotting but I could not see consistent results in IF
Jaime (Verified Customer) (01-24-2018)