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Vimentin Polyclonal antibody

The Vimentin polyclonal antibody (10366-1-AP) is one of the most highly cited Vimentin antibodies in the market. It is suitable for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA and has been referenced in over 2,603 publications.

Cat No. 10366-1-AP

Publications(2603)

Reviews (12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, IP-MS, ELISA, Cell treatment

Synonyms

VIM

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

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Proteintech Guarantee

Filter: AllWBIHCIF-PIF-FroIF/ICCFC (Intra)

WB analysis using 10366-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat cells using 10366-1-AP
WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded normal colon slide using 10366-1-AP (Vimentin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10366-1-AP (VIM antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human breast cancer using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human kidney using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human breast cancer using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human kidney using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of hiPSC-derived cortical astrocytes (7 DIV) using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed BrainXell hiPSC-derived cortical astrocytes (7 DIV) using antibodies against GFAP and Vimentin (10366-1-AP) at a dilution of 1:200, 1:400, or 1:500. Data provided by BrainXell. iPSC-Derived Human Neurons and Glia from BrainXell - BrainXell
IF Staining of HepG2 using 10366-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 10366-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (10366-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 10366-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Vimentin (10366-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of mMSCs using 10366-1-AP
1x10^6 mMSCs cells were intracellularly stained with 0.5 ug Vimentin Polyclonal antibody (10366-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2603)
Protocols
Reviews (12)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, Jurkat cells, HeLa cells
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human breast cancer tissue, human kidney tissue
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	HepG2 cells, hiPSC-derived cortical astrocytes (7 DIV)
Positive FC (Intra) detected in	HeLa cells, mMSCs cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, IP-MS, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, monkey, chicken, bovine, hamster, goat, deer
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0489 Product name: Recombinant human Vimentin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-466 aa of BC000163 Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE Predict reactive species
Full Name	vimentin
Calculated Molecular Weight	466 aa, 54 kDa
Observed Molecular Weight	54 kDa
GenBank Accession Number	BC000163
Gene Symbol	VIM
Gene ID (NCBI)	7431
ENSEMBL Gene ID	ENSG00000026025
RRID	AB_2273020
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08670
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Product Specific Protocols
FC protocol for Vimentin antibody 10366-1-AP	Download protocol
IF protocol for Vimentin antibody 10366-1-AP	Download protocol
IHC protocol for Vimentin antibody 10366-1-AP	Download protocol
WB protocol for Vimentin antibody 10366-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	IF	Nature iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion. Authors - Fengxiang Wang View Article
human	WB	J Hematol Oncol LincK contributes to breast tumorigenesis by promoting proliferation and epithelial-to-mesenchymal transition. Authors - Jing Li View Article
mouse	IF	J Hematol Oncol Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers. Authors - Ming Yi View Article
human	IF	Cell A transcription factor atlas of directed differentiation Authors - Julia Joung View Article
human	FC (Intra)	Cell Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma Authors - Abhirami Visvanathan View Article
human	IHC	Mol Cancer circNOX4 activates an inflammatory fibroblast niche to promote tumor growth and metastasis in NSCLC via FAP/IL-6 axis Authors - Yan Zhao View Article

Reviews

4.5 out of 5

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12 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Qin (Verified Customer) (09-08-2026)	very good antibody for Vimentin WB Applications: Western Blot
Sama (Verified Customer) (03-26-2026)	Very beautiful staining - very strong staining of vimentin+ ependymal cell processes Applications: Immunofluorescence Primary Antibody Dilution: 1:2500 Cell Tissue Type: Mouse brain slice Vimentin Antibody Immunofluorescence validation (1:2500 dilution) in Mouse brain slice (Cat no:10366-1-AP)
Vernon (Verified Customer) (01-29-2024)	Western blot signal is great with 30 sec exposure secondary antibody 1:5000 1hr. at room temp Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Ewing sarcoma cell line Vimentin Antibody Western Blot validation (1:2000 dilution) in Ewing sarcoma cell line (Cat no:10366-1-AP)
Tobias (Verified Customer) (06-16-2023)	Works well in IF Applications: Immunofluorescence Primary Antibody Dilution: 1:200
Emma (Verified Customer) (02-22-2022)	Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval). Applications: Immunofluorescence Primary Antibody Dilution: 1:50 Cell Tissue Type: Prostate
Yuki (Verified Customer) (01-07-2022)	Consistently works antibody Applications: Immunofluorescence Primary Antibody Dilution: 1:150 Cell Tissue Type: gut duct tissue Vimentin Antibody Immunofluorescence validation (1:150 dilution) in gut duct tissue (Cat no:10366-1-AP)
Hala (Verified Customer) (07-08-2021)	Works veryyyy gooddd Applications: Western Blot Primary Antibody Dilution: 1/1000 - 1/2000 Cell Tissue Type: hepG2 transduced with TDO2 gene, liver cancer cells
Hala (Verified Customer) (04-11-2021)	very good antibody, works very well Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: hepG2 transduced with TDO2 gene Vimentin Antibody Western Blot validation (1/1000 dilution) in hepG2 transduced with TDO2 gene (Cat no:10366-1-AP)
wei (Verified Customer) (04-27-2020)	I tried in C2C12 cells Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: C2C12 Vimentin Antibody Western Blot validation (1:1000 dilution) in C2C12 (Cat no:10366-1-AP)
Patricia (Verified Customer) (02-25-2020)	Works well. Applications: Immunofluorescence, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Ovarian cancer
Kishor (Verified Customer) (01-30-2019)	Good results in western blotting but I could not see consistent results in IF Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: H69, HUCCT-1
Jaime (Verified Customer) (01-24-2018)	Applications: Immunofluorescence Primary Antibody Dilution: 1/200 Cell Tissue Type: Mouse ventricle frozen sections Vimentin Antibody Immunofluorescence validation (1/200 dilution) in Mouse ventricle frozen sections (Cat no:10366-1-AP)