• Featured Product
  • KD/KO Validated

Vimentin Antibody

Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10366-1-AP

Synonyms

VIM, vimentin



Tested Applications

Positive WB detected inHeLa cells, A549 cells, Raji cells, Jurkat cells, mouse heart tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human tonsillitis tissue, human ovary tumor tissue, human liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10366-1-AP targets Vimentin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, sika deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489
Full Name vimentin
Calculated molecular weight 466aa,54 kDa
Observed molecular weight 54 kDa
GenBank accession number BC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Product Specific Protocols
WB protocol for Vimentin antibody 10366-1-APDownload protocol
IHC protocol for Vimentin antibody 10366-1-APDownload protocol
IF protocol for Vimentin antibody 10366-1-APDownload protocol
FC protocol for Vimentin antibody 10366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Sci Monit

PMID: 30605443

Authors - Lin Chen
humanWB

Cytotechnology

Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress Slug expression.

Authors - Haijun Fan
humanWB

Cancer Sci

Nuclear factor I/B promotes colorectal cancer cell proliferation, epithelial-mesenchymal transition and 5-fluorouracil resistance.

Authors - ZhengYi Liu
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
sika deerWB

J Proteomics

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Authors - Zhen Dong
mouseWB,IHC

J Exp Clin Cancer Res

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Authors - Chunfeng Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

wei (Verified Customer) (04-27-2020)

I tried in C2C12 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12
Vimentin Antibody Western Blot validation (1:1000 dilution) in C2C12 (Cat no:10366-1-AP)
FH

Patricia (Verified Customer) (02-25-2020)

Works well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer
FH

Kishor (Verified Customer) (01-30-2019)

Good results in western blotting but I could not see consistent results in IF

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Jaime (Verified Customer) (01-24-2018)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Mouse ventricle frozen sections
Vimentin Antibody Immunofluorescence validation (1/200 dilution) in Mouse ventricle frozen sections (Cat no:10366-1-AP)