VIP Polyclonal antibody

VIP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16233-1-AP

Synonyms

PHM27, vasoactive intestinal peptide, VIP, VIP peptides



Tested Applications

Positive IHC detected inhuman colon tissue, human small intestine tissue, rat small intestine tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

16233-1-AP targets VIP in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VIP fusion protein Ag8933
Full Name vasoactive intestinal peptide
Calculated molecular weight 169 aa, 19 kDa
GenBank accession numberBC009794
Gene symbol VIP
Gene ID (NCBI) 7432
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for VIP antibody 16233-1-APDownload protocol
IF protocol for VIP antibody 16233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

PMID: 31509750

Authors - Jing Xue