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VPS16 Polyclonal antibody

VPS16 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17776-1-AP

Synonyms

hVPS16, VPS16



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17776-1-AP targets VPS16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS16 fusion protein Ag12202
Full Name vacuolar protein sorting 16 homolog (S. cerevisiae)
Calculated molecular weight 839 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC073959
Gene symbol VPS16
Gene ID (NCBI) 64601
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS16 antibody 17776-1-APDownload protocol
IHC protocol for VPS16 antibody 17776-1-APDownload protocol
IP protocol for VPS16 antibody 17776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biochim Biophys Acta Mol Cell Res

Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes.

Authors - Jinni Hong

Am J Physiol Cell Physiol

Control of Lysosomal TRPML1 Channel Activity and Exosome Release by Acid Ceramidase in Mouse Podocytes.

Authors - Guangbi Li
humanWB, IF

J Biol Chem

Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.

Authors - Rik van der Kant
humanIF

J Cell Sci

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Authors - van der Kant Rik R
ratWB

Cell Death Dis

Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.

Authors - Lixuan Zhan
humanWB

Curr Biol

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Authors - Anna Ulbricht