WEE1 Polyclonal antibody
WEE1 Polyclonal Antibody for ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 14375-1-AP
Synonyms
Validation Data Gallery
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
14375-1-AP targets WEE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WEE1 fusion protein Ag5751 |
| Full Name | WEE1 homolog (S. pombe) |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC051831 |
| Gene Symbol | WEE1 |
| Gene ID (NCBI) | 7465 |
| RRID | AB_2878049 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway.
| ||
Oncogene miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells. | ||
Exp Cell Res NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation. | ||
Exp Cell Res The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation. | ||
Nanomedicine The enhancement of siPLK1 penetration across BBB and its anti glioblastoma activity in vivo by magnet and transferrin co-modified nanoparticle. |
