|Positive WB detected in||HeLa cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14375-1-AP targets WEE1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WEE1 fusion protein Ag5751|
|Full Name||WEE1 homolog (S. pombe)|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||72 kDa|
|GenBank accession number||BC051831|
|Gene ID (NCBI)||7465|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cancer Manag Res
miR-526b-3p serves as a prognostic factor and regulates the proliferation, invasion, and migration of glioma through targeting WEE1.
The enhancement of siPLK1 penetration across BBB and its anti glioblastoma activity in vivo by magnet and transferrin co-modified nanoparticle.
Exp Cell Res
Cdc14a has a role in spermatogenesis, sperm maturation and male fertility.
miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.