YAP1 Antibody 16 Publications

Rabbit Polyclonal| Catalog number: 13584-1-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

Be the first to review this product


-_-

Freight/Packing

Con: 45 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, zebrafish

Positive WB detected in:
HeLa cells, HeLa cells, MCF-7 cells

Positive IP detected in:
NIH/3T3 cells

Positive IHC detected in:
human ovary tumor tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
NIH3T3 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Yes-associated protein 1, 65kDa

Calculated molecular weight:
504aa,54 kDa

Observed molecular weight:
65 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
YAP1

Synonyms
65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog
Background

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).This antibody is a rabbit polyclonal antibody. The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65kDa. (PMID: 26695440)


Back
to top