• Featured Product
  • KD/KO Validated

YAP1 Antibody

YAP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 13584-1-AP

Synonyms

65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inNIH3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13584-1-AP targets YAP1 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, zebrafish samples.

Reactivity human, mouse, zebrafish
Cited Species chicken, human, monkey, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YAP1 fusion protein Ag4510
Full Name Yes-associated protein 1, 65kDa
Calculated molecular weight 504aa,54 kDa
Observed molecular weight 65 kDa
GenBank accession number BC038235
Gene symbol YAP1
Gene ID (NCBI) 10413
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).This antibody is a rabbit polyclonal antibody. The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65kDa. (PMID: 26695440)

Protocols

Product Specific Protocols
WB protocol for YAP1 antibody 13584-1-APDownload protocol
IHC protocol for YAP1 antibody 13584-1-APDownload protocol
IF protocol for YAP1 antibody 13584-1-APDownload protocol
IP protocol for YAP1 antibody 13584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Elife

Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling.

Authors - Kerim B Kaylan
zebrafishIHC

Curr Biol

The Hippo Pathway Regulates Caveolae Expression and Mediates Flow Response via Caveolae.

Authors - Valentina Rausch
chickenWB,IHC

RNA Biol

Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition.

Authors - Xinheng Zhang
humanWB,IF

Onco Targets Ther

Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1.

Authors - Jianlin Wang
  • KD Validated
mouseIF

J Hepatol

F-actin dynamics regulates mammalian organ growth and cell fate maintenance.

Authors - Arianna Pocaterra
humanWB

Cells

Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.

Authors - Minjing Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Arianna Pocaterra (03-01-2019)

Genetically validated on YAP-null liver sections.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Liver tissue
No image submitted with review
FH

Joshua Morgan (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with DAPI and phalloidin. Stain was mix of nuclear, cytosolic, and junctional.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MDCK Canine Kidney Epithelial
YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in MDCK Canine Kidney Epithelial (Cat no:13584-1-AP)
FH

Joshua Morgan (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of nuclear, junctional, and cytosolic.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MDCK Canine Kidney Epithelial
YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in MDCK Canine Kidney Epithelial (Cat no:13584-1-AP)
FH

Juan Pablo Zanin (11-29-2018)

CGN treated with Shh (1ug/ml) for 48hs to induced proliferation, I see a nice induction of YAP1 I get a nice clean band

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Culture of Granule cell neurons from P7 rat pups
YAP1 Antibody Western Blot, validation (1:1000 dilution) in Culture of Granule cell neurons from P7 rat pups (Cat no:13584-1-AP)
FH

kk Qin (11-21-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human PANC1 cells
No image submitted with review